RNAseq de novo assembly

RNAseq de novo assembly (12/02/2019)

The Sigenae team organizes a training course for bio-informaticians and biologists aiming at introduce analysis of sequences. It focuses on de novo assembly (without reference genome) of reads produced by ‘RNA-Seq’. This training session is designed to introduce sequences from ‘NGS’ (Next Generation Sequencing) and particularly Illumina platforms (HiSeq). You will use our RNA-Seq assembly pipeline called DRAP, learn how to assemble distinct samples, how to assemble multiple samples and how to assess the quality of your assemblies.



This training is focused on practice. It consists of 4 modules with a large variety of exercises:

  • Introduction (09:00 am to 10:00 am): introduction to de novo assembly and analysis of RNA-seq data.
  • The DRAP software (10:00 am to 12:00 pm): Pipeline description and runDrap usage.
  • The DRAP software (14:00 am to 16:00 pm): runMeta and runAssessment usage.
  • Discussion (16:00 am to 17:00 pm): discussion about results and assembly quality


The session will take place in the room ‘salle de formation’ at INRA center of Toulouse-Auzeville.


  Prerequisites: ability to use a Unix environment (see Unix training).


Training slides and exercises:

training slides and exercises

training slides


Bookings: RNAseq de novo assembly

Bookings have closed for this event.